Direct Regeneration from Leaf Disc Explants of Peanut: Grafting Improves Survival Rate

Mahmood ul Hassan; Zahid Akram; Farhat Nazir; Noreen Fatima; Yusuf Zafar; Zaheer Ahmad; Zahid Hussain Shah; Khurram Shahzad; Mahpara Fatima; Madiha Zaynab

نویسندگان

Mahmood ul Hassan Department of Plant Breeding and Genetics, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi-Pakistan.
Zahid Akram Department of Plant Breeding and Genetics, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi-Pakistan.
Farhat Nazir National Institute for Genomics and Advanced Biotechnology, NARC, Park Road, Islamabad-Pakistan.
Noreen Fatima Department of Plant Breeding and Genetics, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi-Pakistan.
Yusuf Zafar National Institute for Genomics and Advanced Biotechnology, NARC, Park Road, Islamabad-Pakistan.
Zaheer Ahmad Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad-Pakistan.
Zahid Hussain Shah Department of Plant Breeding and Genetics, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi-Pakistan.
Khurram Shahzad Department of Plant Breeding and Genetics, University of Haripur, Pakistan.
Mahpara Fatima College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
Madiha Zaynab College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.

کلمات کلیدی:

Grafting, In vitro regeneration, Leaf disc, Naphthalene acetic acid, peanut.

چکیده

Leaf discs of 0.5 cm diameter from 10 days old seedlings of four peanut cultivars were cultured on Murashige and Skoog (MS) medium supplemented with twelve different combinations of Thidiazuron (TDZ) and Naphthalene acetic acid (NAA). The highest number of responding explants (52.67%) with the highest number of shoot buds (5.65) per responding explant was achieved at a combination of 0.5 mg/l NAA and 0.5 mg/l TDZ. The explants were then shifted to fresh MS medium containing 8 mg/l Benzylaminopurine (BAP) and 0.5 mg Indole Acetic Acid (IAA) for shoot elongation. Root induction was highest (56.07%) at half-strength MS medium supplemented with 1.5 mg/l NAA. Grafting of in vitro regenerated shoots on 10 days old seedling rootstock showed highest (61.30%) success rate. The survival rate of plants obtained from grafting was almost double (45.76%) than those achieved by rooting of shoots (22.41%). There was significant variation among four varieties regarding the number of responding explant, the number of shoots per explant, rooting or grafting percentage and survival rate. Golden proved to be the best variety in terms of the number of plants reaching maturity.