Draft Genomes Sequences of KPC-2, OXA-1, OXA-9, SHV-28, TEM-1 and CTX-M-15 Producing Klebsiella pneumoniae ST15 and KPC-2, OXA-1, TEM-1 and LEN-2 Producing Klebsiella variicola ST32 from Clinical Samples in Rio de Janeiro, Brazil
Keywords:
Genome, antibiotic resistance, infection, Klebsiella pneumonia, Klebsiella variicola.Abstract
KPC-producing bacteria are considered one of the most worrisome multidrug-resistant micro-organisms in nosocomial infections. Here we report the draft genomes sequences of two KPC-2 and CTX-M-15 producing Klebsiella pneumoniae sequence type 15 (ST15) and Klebsiella variicola sequence type 32 (ST32) isolates obtained from clinical samples of patients in Rio de Janeiro. A genomic library was constructed using a Nextera XT Kit. An Illumina platform was used to perform whole-genome sequencing (WGS). WGS of isolates K. pneumoniae and K. variicola resulted in estimated genome sizes of 5662554 and 5868756 bp, respectively. Resistome analysis of the clinical strains revealed the presence of resistance genes to the following antimicrobials in K. pneumoniae: aminoglycosides [aadA1], [aph (3’) - Ia], [aph (3'')-Ib], [aac (6')-Ib3], [aadA2] and [aph(6)-Id]; beta-lactams (blaOXA-1, blaoxa-9, blaCTX-M-15, blaTEM-1, and blaSHV-28); carbapenem (blaKPC-2); fluoroquinolones [aac (60) – Ib – cr], [oqxA] and [oqxB]; fosfomycin [fosA]; macrolides [mph(A)] and [erm(B)]; phenicols [catB3]; sulfonamides [sul1]; trimethoprim [dfrA12] and tetracycline [tetA] and to K. variicola: aminoglycosides [aadA1], [aph (3)-IIa], [aac(6')-Ib-cr] and [aph(6) - Id]; beta lactams (blaOXA-1, blaCTX - M - 15, blaTEM-1B and blaLEN - 2); carbapenem (blaKPC-2); fluoro-
quinolones [aac (60)-Ib-cr], [qnrB1], [oqxA] and [oqxB]; fosfomycin [fosA]; phenicols [catB3]; sulfonamides [sul2]; trimethoprim [dfrA14] and tetracycline [tetA]. The resistome revealed by this study might be a useful tool to elucidate the dissemination of nosocomial resistance genes in Rio de Janeiro.
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