Validation of the Ethnopharmacological Uses of Withania somnifera


  • Muhammad Shuaib Institute School of Ecology and Environmental Science, Yunnan University, NO.2 North Cuihu road, Kunming, Yunnan, 650091, PR. China.
  • Sajjad Ali Department of Botany, Bacha Khan University, Charsadda, Pakistan.
  • Kashif Ali Institute of Ecology and Geobotany, School of Ecology and Environmental Science, Yunnan University, No.2 North Cuihu Road, Kunming, Yunnan, 650091, PR. China.
  • Firasat Hussain Yunnan Institute of Microbiology, School of Life Science, Yunnan University, Kunming, 650091, PR China.
  • Muhammad Ilyas Department of Entomology, The University of Agriculture Peshawar, Pakistan.
  • Muhammad Arif Department of Microbiology, Abasyn University Peshawar, Pakistan.
  • Fida Hussain Department of Botany, Qurtaba University Peshawar, Pakistan.


Antibacterial, Antifungal, activity, W. Somnifera, leaves, extracts.


The antimicrobial potential of some solvent extracts of Withania somnifera leaves against different strains of bacteria and fungi was investigated. The leaves of W. somnifera species were collected from Bacha Khan University, Charsadda, Pakistan, rinsed with distilled water and extracted with chloroform and ethanol. Antibacterial activity of crude extracts of W. somnifera was determined by agar well diffusion method against gram positive, gram negative bacterial and antifungal activity was determined by agar diffusion method against fungal strains. All tested plant extracts showed varying zones of inhibition against bacteria and fungi tested. The highest zone of inhibition of 27 mm diameter was noted in S. aureus with ethanol extract while the lowest 7 mm zone of inhibition was reported in Klebsiella pneumonia. Among the fungal species, promising level of antifungal activity was observed in all fractions against A. flavus and A. niger. The results obtained exhibit that this plant has good medicinal potential, and it needs further phytochemical exploitation to isolate phytochemical constituents having antibacterial and antifungal activities.




How to Cite

Shuaib, M., Ali, S., Ali, K., Hussain, F., Ilyas, M., Arif, M., & Hussain, F. (2019). Validation of the Ethnopharmacological Uses of Withania somnifera. International Journal of Nanotechnology and Allied Sciences, 3(1), 1–6. Retrieved from




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