Biosynthesis of Penicillin Encoded by Gene acvA UniRef90_A2QZ81 present in Circular DNA Sequence of Aspergillus salvadorensis

Abstract

The main objective was to determine penicillin-producing enzymes. The analysis was based on the extraction of gDNA, qPCR, cDNA from Aspergillus salvadorensis that was carried out in 2024 at MACROGEN INC by Metagenome Shotgun Sequencing Reports Illumina, the entire sequence was analyzed in 2025 to determine enzymes, proteins and secondary metabolites in their genetics and identify the acvA gene. Strains 10 of Staphylococcus aureus and 10 Escherichia coli were inoculated in Muller Hinton dishes. Then, 0.01 microgram spores of the A. salvadorensis strain were inoculated in the center, incubated at 36 °C x 4 days. Tukey's test it was used to determine significant differences between species. MACROGEN reports by sequencing UniRef90_A2QZ81 that it has biosynthesis properties of penicillin catalyzed by the enzyme encoded acvA with a found frequency of 31.7269 in A. salvadorensis. Growth of the fungus forming a halo of inhibition in the plates between 1 a 4 mm inoculated with grampositive and gramnegative bacteria constitutes solid preliminary evidence of antibacterial activity associated with bioactive metabolites. The production of penicillinase makes Aspergillus salvadorensis an organism capable of interfering with antimicrobial susceptibility tests, selective media, and microbial interaction experiments. Its ability to inactivate penicillins significantly modifies the behavior of susceptible bacterial populations, allowing their survival, proliferation and expansion of the fungus under conditions that would normally inhibit them. Tukey's test was applied to compare the means of the two treatments studied. The difference observed between the means was 0.006. It is concluded that there is no significant difference between treatments. This result is consistent with the ANOVA, which also showed a non-significant difference between the species.